We wish to thank Dr. Stephen J. Benkovic and Dr. Dan Harki for their helpful editorial suggestions and the Univ. Minnesota Endowment Fund for financial support.
Reversible Re-programing of Cell–Cell Interactions†
Article first published online: 2 APR 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 126, Issue 20, pages 5212–5216, May 12, 2014
How to Cite
Gabrielse, K., Gangar, A., Kumar, N., Lee, J. C., Fegan, A., Shen, J. J., Li, Q., Vallera, D. and Wagner, C. R. (2014), Reversible Re-programing of Cell–Cell Interactions. Angew. Chem., 126: 5212–5216. doi: 10.1002/ange.201310645
- Issue published online: 8 MAY 2014
- Article first published online: 2 APR 2014
- Manuscript Revised: 17 FEB 2014
- Manuscript Received: 8 DEC 2013
- Univ. Minnesota Endowment Fund
- Chimäre Antigenrezeptoren;
The ability to engineer and re-program the surfaces of cells would provide an enabling synthetic biological method for the design of cell- and tissue-based therapies. A new cell surface-engineering strategy is described that uses lipid-chemically self-assembled nanorings (lipid-CSANs) that can be used for the stable and reversible modification of any cell surface with a molecular reporter or targeting ligand. In the presence of a non-toxic FDA-approved drug, the nanorings were quickly disassembled and the cell–cell interactions reversed. Similar to T-cells genetically engineered to express chimeric antigen receptors (CARS), when activated peripheral blood mononuclear cells (PBMCs) were functionalized with the anti-EpCAM-lipid-CSANs, they were shown to selectively kill antigen-positive cancer cells. Taken together, these results demonstrate that lipid-CSANs have the potential to be a rapid, stable, and general method for the reversible engineering of cell surfaces and cell–cell interactions.