Work on sortase A in the laboratory of O.S. is supported by a grant from the National Institute of Allergy and Infectious Diseases (AI038897).
Total Chemical Synthesis of the Enzyme Sortase AΔN59 with Full Catalytic Activity†
Article first published online: 25 MAR 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 126, Issue 18, pages 4750–4754, April 25, 2014
How to Cite
Deng, F.-K., Zhang, L., Wang, Y.-T., Schneewind, O. and Kent, S. B. H. (2014), Total Chemical Synthesis of the Enzyme Sortase AΔN59 with Full Catalytic Activity. Angew. Chem., 126: 4750–4754. doi: 10.1002/ange.201310900
- Issue published online: 25 APR 2014
- Article first published online: 25 MAR 2014
- Manuscript Received: 16 DEC 2013
- National Institute of Allergy and Infectious Diseases. Grant Number: AI038897
- Chemische Ligation;
- Chemische Proteinsynthesen;
- Konvergente Synthesen;
- Sortase A
The enzyme sortase A is a ligase which catalyzes transpeptidation reactions.1, 2 Surface proteins, including virulence factors, that have a C terminal recognition sequence are attached to Gly5 on the peptidoglycan of bacterial cell walls by sortase A.1 The enzyme is an important anti-virulence and anti-infective drug target for resistant strains of Gram-positive bacteria.2 In addition, because sortase A enables the splicing of polypeptide chains, the transpeptidation reaction catalyzed by sortase A is a potentially valuable tool for protein science.3 Here we describe the total chemical synthesis of enzymatically active sortase A. The target 148 residue polypeptide chain of sortase AΔN59 was synthesized by the convergent chemical ligation of four unprotected synthetic peptide segments. The folded protein molecule was isolated by size-exclusion chromatography and had full enzymatic activity in a transpeptidation assay. Total synthesis of sortase A will enable more sophisticated engineering of this important enzyme molecule.