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Review
Liquid Column Chromatography with Chemical Derivatizations after Separation. [New analytical methods (15)]
Article first published online: 22 DEC 2003
DOI: 10.1002/anie.197901801
Copyright © 1979 by Verlag Chemie, GmbH, Germany
Issue
Angewandte Chemie International Edition in English
Volume 18, Issue 3, pages 180–186, March 1979
Additional Information
How to Cite
Schwedt, G. (1979), Liquid Column Chromatography with Chemical Derivatizations after Separation. [New analytical methods (15)]. Angew. Chem. Int. Ed. Engl., 18: 180–186. doi: 10.1002/anie.197901801
Publication History
- Issue published online: 22 DEC 2003
- Article first published online: 22 DEC 2003
- Manuscript Received: 23 MAY 1978
- Abstract
- References
- Cited By
Keywords:
- Liquid chromatography;
- Column chromatography;
- Separation;
- Analytical methods;
- HPLC (high pressure liquid chromatography)
Abstract
Modern liquid column chromatography (high-pressure liquid chromatography, HPLC) has evolved in the last few years into a highly efficient and versatile separation technique. The selectivity of an analytical process that depends upon a previous separation step can in many cases be increased considerably by chemical derivatizations after the separation. In addition, lower detection limits can be achieved in this way than in detection without derivatization. The physicochemical principles of these combined processes involving chromatographic separation and chemical derivatization prior to detection (coupling of HPLC and a reaction detector) are presented and discussed. The state of development is outlined, with a survey of the more important applications so far described in the literature.