Recombinant Whole Cells as Catalysts for the Enzymatic Synthesis of Oligosaccharides and Glycopeptides

Authors

  • Guido F. Herrmann,

    1. Department of Chemistry, The Scripps Research Institute, 10666 North Torrey Pines Road, La Jolla, CA 92037 (USA), Telefax: Int. code + (619)554 6731
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  • Peng Wang,

    1. Department of Chemistry, The Scripps Research Institute, 10666 North Torrey Pines Road, La Jolla, CA 92037 (USA), Telefax: Int. code + (619)554 6731
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  • Gwo-Jenn Shen,

    1. Department of Chemistry, The Scripps Research Institute, 10666 North Torrey Pines Road, La Jolla, CA 92037 (USA), Telefax: Int. code + (619)554 6731
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  • Chi-Huey Wong

    Corresponding author
    1. Department of Chemistry, The Scripps Research Institute, 10666 North Torrey Pines Road, La Jolla, CA 92037 (USA), Telefax: Int. code + (619)554 6731
    • Department of Chemistry, The Scripps Research Institute, 10666 North Torrey Pines Road, La Jolla, CA 92037 (USA), Telefax: Int. code + (619)554 6731
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  • This research was supported by the National Institutes of Health (GM 44154). G. F. H. thanks the German Academic Exchange Service for financial support (Doktorandenstipendium HSP II/AUFE).

Abstract

An easier, faster, cheaper approach to the enzymatic synthesis of oligosaccharides would be to employ whole cells rather than purified enzymes. In this report recombinant E. coli cells mediate the mannosylation of monosaccharides and glycopeptides. It was also proven that the relevant enzyme, mannosyl transferase, does not leak from the periplasmic space of the cells into the medium.

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