SEARCH

SEARCH BY CITATION

Keywords:

  • copper compounds;
  • EPR spectroscopy;
  • metalloproteins;
  • mutagenesis;
  • Raman spectroscopy

Azide, cyanide, and thiocyanate are the anions that, on addition to a variant produced by site-directed mutagenesis, Met121Gly, of Pseudomonas aeruginosa azurin, produce unprecedented copper proteins with markedly different spectroscopic properties. This ability to generate ligand-accessible metal centers in coordinatively saturated metalloproteins by directed mutagenesis might possibly bring the synthesis of metalloproteins that can be used as ion sensors a step nearer.