Novel Biological Copper Proteins through Anion Addition to the Mutant Met121Gly of Pseudomonas aeruginosa Azurin


  • This work was supported by the University of Houston and the American Cyanamid (Faculty Research Award to J. P. G.). The authors express their gratitude to Prof. R. S. Czernuszewicz and G. Fraczkiewicz (University of Houston) for the resonance Raman spectra, and Mr. V. Kurchev for assistance in acquiring the EPR spectra.


Azide, cyanide, and thiocyanate are the anions that, on addition to a variant produced by site-directed mutagenesis, Met121Gly, of Pseudomonas aeruginosa azurin, produce unprecedented copper proteins with markedly different spectroscopic properties. This ability to generate ligand-accessible metal centers in coordinatively saturated metalloproteins by directed mutagenesis might possibly bring the synthesis of metalloproteins that can be used as ion sensors a step nearer.