This work was supported by the Deutsche Forschungsgemeinschaft (W. K.), the Fonds der Chemischen Industrie (W. K.), and the Studienstiftung des Deutschen Volkes (A. S.). We thank Dr. V. Sinnwell and Dr. C. Schulze (Universität Hamburg) for recording the NMR and ESI spectra, respectively. dTDP = thymidine-5′-diphosphate.
Synthesis of dTDP-6-Deoxy-4-ketoglucose and Analogues with Native and Recombinant dTDP-Glucose-4,6-dehydratase†
Article first published online: 22 DEC 2003
Copyright © 1995 by VCH Verlagsgesellschaft mbH, Germany
Angewandte Chemie International Edition in English
Volume 34, Issue 16, pages 1748–1749, September 1, 1995
How to Cite
Stein, A., Kula, M.-R., Elling, L., Verseck, S. and Klaffke, W. (1995), Synthesis of dTDP-6-Deoxy-4-ketoglucose and Analogues with Native and Recombinant dTDP-Glucose-4,6-dehydratase. Angew. Chem. Int. Ed. Engl., 34: 1748–1749. doi: 10.1002/anie.199517481
- Issue published online: 22 DEC 2003
- Article first published online: 22 DEC 2003
- Manuscript Received: 17 MAR 1995
The enzymatic deoxygenation of dTDP-glucose and two 3-deoxy analogues [Eq. (a)] is now possible on a preparative scale with dTDP-D-glucose-4,6-dehydratase. In addition a highly selective HPLC method for the separation of structurally related activated sugars has been developed. This should provide new impetus for structure–function studies of deoxysugars and further elucidation of their biosynthetic pathways. R OH, H, N3; dTDP = thymidine-5′-diphosphate.