Enzyme-Catalyzed Reactions, Part 23. This work was supported by the Bundesministerium für Bildung und Forschung and by the Fonds der Chemischen Industrie. Part 22: F. Effenberger, S. Heid, Tetrahedron Asymmetry1995, 6, 2945–2952.
Article first published online: 22 DEC 2003
Copyright © 1996 by VCH Verlagsgesellschaft mbH, Germany
Angewandte Chemie International Edition in English
Volume 35, Issue 4, pages 437–439, March 1, 1996
How to Cite
Förster, S., Roos, J., Effenberger, F., Wajant, H. and Sprauer, A. (1996), The First Recombinant Hydroxynitrile Lyase and its Application in the Synthesis of (S)-Cyanohydrins. Angew. Chem. Int. Ed. Engl., 35: 437–439. doi: 10.1002/anie.199604371
Dedicated to Professor Manfred Regitz on the occasion of his 60th birthday
- Issue published online: 22 DEC 2003
- Article first published online: 22 DEC 2003
- Manuscript Received: 11 OCT 1995
- asymmetric syntheses;
- enzymatic catalysis;
The cassava species Manihot esculenta provided the first recombinant hydroxynitrile lyase (MeHNL) that became accessible by overexpression in E. coli. This MeHNL catalyzes the enantioselective addition of HCN (depicted below) not only to aliphatic, aromatic, and heteroaromatic aldehydes, but also to ketones to give (S)-cyanohydrins in high optical yields. R alkyl, aryl, heteroaryl; R1 H, CH3.