Magnetization Studies of the Reduced Active Form of the Catalase from Thermus thermophilus

Authors

  • Lilian Jacquamet,

    1. Départment de Recherche Fondamentale sur la Matiére Condensée Service de Chimie Inorganique et Biologique Laboratoire de Chimie de Coordination (Unité de Recherche Associée au CNRS no. 1194) CEA-Grenoble, F.38054 Grenoble Cedox 9 (France)Fax: Int. Code+47688 50 90, e-mail:jlatour@cea.fr
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  • Dr. Isabelle Michaud-Soret,

    1. Départment de Recherche Fondamentale sur la Matiére Condensée Service de Chimie Inorganique et Biologique Laboratoire de Chimie de Coordination (Unité de Recherche Associée au CNRS no. 1194) CEA-Grenoble, F.38054 Grenoble Cedox 9 (France)Fax: Int. Code+47688 50 90, e-mail:jlatour@cea.fr
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  • Dr. Noële Debaecker-Petit,

    1. Départment de Recherche Fondamentale sur la Matiére Condensée Service de Chimie Inorganique et Biologique Laboratoire de Chimie de Coordination (Unité de Recherche Associée au CNRS no. 1194) CEA-Grenoble, F.38054 Grenoble Cedox 9 (France)Fax: Int. Code+47688 50 90, e-mail:jlatour@cea.fr
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  • Dr. Jean-Marc Latour,

    Corresponding author
    1. Départment de Recherche Fondamentale sur la Matiére Condensée Service de Chimie Inorganique et Biologique Laboratoire de Chimie de Coordination (Unité de Recherche Associée au CNRS no. 1194) CEA-Grenoble, F.38054 Grenoble Cedox 9 (France)Fax: Int. Code+47688 50 90, e-mail:jlatour@cea.fr
    • Départment de Recherche Fondamentale sur la Matiére Condensée Service de Chimie Inorganique et Biologique Laboratoire de Chimie de Coordination (Unité de Recherche Associée au CNRS no. 1194) CEA-Grenoble, F.38054 Grenoble Cedox 9 (France)Fax: Int. Code+47688 50 90, e-mail:jlatour@cea.fr
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  • Dr. Vladimir V. Barynin,

    1. Institute of Crystallography, Russian Academy of Sciences Moscow (Russia)
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  • Dr. Jean-Luc Zimmermann

    1. Department of Molecular and Cellular Biology CEA/Saclay (France)
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Abstract

original image

The presence of an aqua bridge in the active and phosphate-bound reduced form of manganese-containing catalases is the key finding from magnetization studies on these compounds. In addition several observations indicate that the phosphate group may form a second bridge. which suggests that two syn coordination sites of the active dimanganese center are occupied by easily exchangeable ligands, namely water molecules. The proposed structures are depicted on the right.

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