Merocyanines as Extremely Bathochromically Absorbing Chromophores in the Halobacterial Membrane Protein Bacteriorhodopsin

Authors

  • Priv.-Doz. Dr. Wolfgang Gärtner,

    Corresponding author
    1. Max-Planck-Institut für Strahlenchemie, Postfach 101365, D-45413 Mülheim an der Ruhr (Germany) Fax: Int. Code +(208)306-3951 e-mail: gaertner@dsa.mpi-muelheim.mpg.de
    • Max-Planck-Institut für Strahlenchemie, Postfach 101365, D-45413 Mülheim an der Ruhr (Germany) Fax: Int. Code +(208)306-3951 e-mail: gaertner@dsa.mpi-muelheim.mpg.de
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  • Prof. Dr. Volker Buss,

    Corresponding author
    1. Fachgebiet Theoretische Chemie der Universität, Lotharstrasse 1, D-47057 Duisburg (Germany) Fax: Int. code +(203)379-2772
    • Fachgebiet Theoretische Chemie der Universität Lotharstrasse 1, D-47057 Duisburg (Germany) Fax: Int. code +(203)379-2772
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  • Dipl.-Chem. Siegfried Steinmüller,

    1. Fachgebiet Theoretische Chemie der Universität, Lotharstrasse 1, D-47057 Duisburg (Germany) Fax: Int. code +(203)379-2772
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  • Prof. Dr. Hans-Dieter Martin,

    Corresponding author
    1. Institut für Organische Chemie and Makromolekulare Chemie der Universität, Universitätsstrasse 1, D-40225 Düsseldorf (Germany) Fax: Int. code +(211)811-1324
    • Institut für Organische Chemie and Makromolekulare Chemie der Universität, Universitätsstrasse 1, D-40225 Düsseldorf (Germany) Fax: Int. code +(211)811-1324
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  • Dr. Dorothee Hoischen

    1. Institut für Organische Chemie and Makromolekulare Chemie der Universität, Universitätsstrasse 1, D-40225 Düsseldorf (Germany) Fax: Int. code +(211)811-1324
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  • This work was supported by the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie. W. G. Thanks Prof. K. Schaffner for generous support and Prof. S. E. Braslavsky for valuable discussions. The proteins were prepared by T. Huestege, and the fluorescence spectra recorded by A. Keil-Block. D. H. and H.-D. M. wish to thank BASF AG for generous gifts of chemicals.

Abstract

original image

Extremely bathochromically absorbing derivatives of the halobacterial membrane protein bacteriorhodopsin(λmax = 570 nm) form upon incorporation of merocyanine dyes of the type shown below into the protein binding site. Upon covalent attachment to the protein (through formation of a protonated Schiff base), these compounds convert in to hemicyanines in which the positive charge is distributed over the entire π system of the chromophore.

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