This work was supported by the Deutsche Forschungsgemeinschaft (DFG), the Bundesministerium für Bildung, Wissesnchaft, Forschung und Technologie (BMBF), and the Fonds der Chemischen Industrie.
Article first published online: 22 DEC 2003
Copyright © 1997 by WILEY-VCH Verlag GmbH, Germany
Angewandte Chemie International Edition in English
Volume 36, Issue 16, pages 1747–1750, September 1, 1997
How to Cite
Müller, A., Henkel, G., Erkens, A., Schneider, K., Müller, A., Nolting, H.-F. and Solé, V. A. (1997), NADH-Induced Changes of the Nickel Coordination within the Active Site of the Soluble Hydrogenase from Alcaligenes eutrophus: XAFS Investigations on Three States Distinguishable by EPR Spectroscopy. Angew. Chem. Int. Ed. Engl., 36: 1747–1750. doi: 10.1002/anie.199717471
Dedicated to Professor Günter Schmid on the occasion of his 60th birthday
- Issue published online: 22 DEC 2003
- Article first published online: 22 DEC 2003
- Manuscript Received: 19 NOV 1996
- EXAFS spectroscopy;
A heterobinuclear Ni-Fe-S center similar to that in the hydrogenase from Desulfovibrio gigas is also present in the considerably more complex soluble hydrogenase from the bacterium Alcaligenes eutrophus, as shown by high-resolution X-ray absorption spectroscopy. Reductive activation of this enzyme with NADH leads to a substantial change in the coordination of the nickel, which is probably due to specific substrate properties. A model for the active site after NADH reduction is shown on the right.