Cubic liquid-crystalline nanoparticles prepared from aqueous dispersions of cubic lipid–water phases are kinetically stable in the presence of certain dispersing agents. The properties of cubic nanoparticles from monoolein–water and other lipid–water systems have been examined with a variety of experimental techniques. The cubic nanoparticles can be stabilized by polymerization of the reactive lipids in cubic lipid assemblies. Several low-energy-input methods have been developed to facilitate the production and application of cubic nanoparticles. The ability to incorporate and deliver lipophilic, amphiphilic, and water-soluble molecules in a controlled manner and the good biocompatibility of cubic nanoparticles make them excellent candidates for drug-delivery applications.