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Immobilization of Natural Products on Glass Slides by Using a Photoaffinity Reaction and the Detection of Protein–Small-Molecule Interactions

Authors

  • Naoki Kanoh Dr.,

    1. Antibiotics Laboratory, Discovery Research Institute, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan, Fax: (+81) 48-462-4669
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  • Saori Kumashiro,

    1. Antibiotics Laboratory, Discovery Research Institute, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan, Fax: (+81) 48-462-4669
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  • Siro Simizu Dr.,

    1. Antibiotics Laboratory, Discovery Research Institute, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan, Fax: (+81) 48-462-4669
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  • Yasumitsu Kondoh Dr.,

    1. Advanced Engineering Center, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351–0198, Japan
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  • Satoru Hatakeyama,

    1. Advanced Engineering Center, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351–0198, Japan
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  • Hideo Tashiro Dr.,

    1. Advanced Engineering Center, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351–0198, Japan
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  • Hiroyuki Osada Dr.

    1. Antibiotics Laboratory, Discovery Research Institute, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan, Fax: (+81) 48-462-4669
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  • We are especially grateful to Fujisawa Pharmaceuticals (Osaka, Japan) for the generous gift of FK506, and to Y. Ichikawa and R. Nakagawa (Bioarchitect Research Group, RIKEN) for DNA sequencing.

Abstract

original image

Library on a glass slide: A variety of small molecules, including complex natural products, are introduced to glass slides using a photoaffinity reaction (see picture). The small molecules retain the inherent ability to interact with the relevant binding proteins. Importantly, this immobilization procedure does not require a specific functional group, and can be applied for use with a diverse small-molecule library.

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