We are grateful for financial support for this work from Acidophil, LLC and intellectual support from Sydney Brenner. V.W.C. is a recipient of a Sloan Foundation Fellowship.
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Communication
Methotrexate Conjugates: A Molecular In Vivo Protein Tag†
Article first published online: 17 MAR 2004
DOI: 10.1002/anie.200352852
Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Miller, L. W., Sable, J., Goelet, P., Sheetz, M. P. and Cornish, V. W. (2004), Methotrexate Conjugates: A Molecular In Vivo Protein Tag. Angew. Chem. Int. Ed., 43: 1672–1675. doi: 10.1002/anie.200352852
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Publication History
- Issue published online: 17 MAR 2004
- Article first published online: 17 MAR 2004
- Manuscript Received: 12 SEP 2003
Keywords:
- bioorganic chemistry;
- dihydrofolate reductase;
- fluorescent probes;
- methotrexate;
- proteins
The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand–receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).