We are grateful for financial support for this work from Acidophil, LLC and intellectual support from Sydney Brenner. V.W.C. is a recipient of a Sloan Foundation Fellowship.
Methotrexate Conjugates: A Molecular In Vivo Protein Tag†
Article first published online: 17 MAR 2004
Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 43, Issue 13, pages 1672–1675, March 19, 2004
How to Cite
Miller, L. W., Sable, J., Goelet, P., Sheetz, M. P. and Cornish, V. W. (2004), Methotrexate Conjugates: A Molecular In Vivo Protein Tag. Angew. Chem. Int. Ed., 43: 1672–1675. doi: 10.1002/anie.200352852
- Issue published online: 17 MAR 2004
- Article first published online: 17 MAR 2004
- Manuscript Received: 12 SEP 2003
- bioorganic chemistry;
- dihydrofolate reductase;
- fluorescent probes;
The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand–receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).