Get access

Enzymatic Behavior by Intercalating Molecules in a Template-Directed Ligation Reaction

Authors

  • Swapan S. Jain,

    1. School of Chemistry and Biochemistry, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA, Fax: (+1) 404-894-2295
    Search for more papers by this author
  • Frank A. L. Anet Dr.,

    1. Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Search for more papers by this author
  • Christopher J. Stahle,

    1. School of Chemistry and Biochemistry, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA, Fax: (+1) 404-894-2295
    Search for more papers by this author
  • Nicholas V. Hud Dr.

    1. School of Chemistry and Biochemistry, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA, Fax: (+1) 404-894-2295
    Search for more papers by this author

  • This work was supported by the Georgia Institute of Technology Office of the Vice Provost for Research. We thank Dr. A. Ellington (University of Texas, Austin) for helpful discussions.

Abstract

original image

Molecular midwifery: A nucleic acid intercalating molecule, proflavine (represented by blue lines), increases the coupling rate of short oligonucleotides in a template-directed synthesis by three orders of magnitude. Several proflavine molecules act cooperatively to function as a concentration-dependent multimolecular “enzyme”.

Get access to the full text of this article

Ancillary