Synthesis and Application of Fluorescence-Labeled Ras-Proteins for Live-Cell Imaging

Authors

  • Reinhard Reents Dr.,

    1. Max-Planck-Institut für molekulare Physiologie, Abteilung Chemische Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund (Gemany) and Universität Dortmund, Fachbereich 3, Organische Chemie, Fax.: (+49) 231-133-2499
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Melanie Wagner Dr.,

    1. Max-Planck-Institut für Molekulare Physiologie, Abteilung Strukturelle Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany, Fax: (+49) 231-133-1435
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Jürgen Kuhlmann Dr.,

    1. Max-Planck-Institut für Molekulare Physiologie, Abteilung Strukturelle Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany, Fax: (+49) 231-133-1435
    Search for more papers by this author
  • Herbert Waldmann Prof. Dr.

    1. Max-Planck-Institut für molekulare Physiologie, Abteilung Chemische Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund (Gemany) and Universität Dortmund, Fachbereich 3, Organische Chemie, Fax.: (+49) 231-133-2499
    Search for more papers by this author

  • This research was supported by the Fonds der Chemischen Industrie, the Max Planck Society, and the Deutsche Forschungsgemeinschaft.

Abstract

original image

Seeing is believing! Semisynthetic Ras-proteins with a fluorophore introduced at an appropriate site have been developed as probes for live-cell imaging experiments. They are processed by the cellular machinery, and their intracellular location can then be determined by confocal laser fluorescence microscopy.

Ancillary