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Keywords:

  • fluorescence;
  • histones;
  • imaging;
  • phosphorylation;
  • reporters
Thumbnail image of graphical abstract

An increase in FRET indicates phosphorylation of histone H3 at serine 28. The protein-based reporter (see picture) responds to phosphorylation through intramolecular complexation between a substrate domain derived from histone H3 and a linked phosphoserine-recognition domain. The reporter is also effective inside living mammalian cells. FRET=fluorescence resonance energy transfer.