We acknowledge support from the NSF MRSEC at the University of Chicago (D.B., grant DMR 0213745). We thank Dr. Neeraj Chopra for helpful discussions.
A One-Pot Total Synthesis of Crambin†
Article first published online: 28 APR 2004
Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 43, Issue 19, pages 2534–2538, May 3, 2004
How to Cite
Bang, D. and Kent, S. B. H. (2004), A One-Pot Total Synthesis of Crambin. Angew. Chem. Int. Ed., 43: 2534–2538. doi: 10.1002/anie.200353540
- Issue published online: 28 APR 2004
- Article first published online: 28 APR 2004
- Manuscript Received: 16 DEC 2003
- native chemical ligation;
- protein engineering;
- protein folding
Making up for lost time: The one-pot synthesis of crambin (see structure) with only a single final purification step gave the target protein of exceptional purity in only two days with an overall yield of ≈40 %. Three unprotected peptide segments were linked by native chemical ligation, and the polypeptide chain assumed its 3D structure without intermediate purification steps.