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Keywords:

  • crambin;
  • native chemical ligation;
  • protein engineering;
  • protein folding
Thumbnail image of graphical abstract

Making up for lost time: The one-pot synthesis of crambin (see structure) with only a single final purification step gave the target protein of exceptional purity in only two days with an overall yield of ≈40 %. Three unprotected peptide segments were linked by native chemical ligation, and the polypeptide chain assumed its 3D structure without intermediate purification steps.