A One-Pot Total Synthesis of Crambin

Authors

  • Duhee Bang,

    1. Institute for Biophysical Dynamics, Department of Biochemistry and Molecular Biology, Department of Chemistry, The University of Chicago, Chicago IL 60637, USA, Fax: (+1) 773-702-0439
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  • Stephen B. H. Kent Prof.

    1. Institute for Biophysical Dynamics, Department of Biochemistry and Molecular Biology, Department of Chemistry, The University of Chicago, Chicago IL 60637, USA, Fax: (+1) 773-702-0439
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  • We acknowledge support from the NSF MRSEC at the University of Chicago (D.B., grant DMR 0213745). We thank Dr. Neeraj Chopra for helpful discussions.

Abstract

original image

Making up for lost time: The one-pot synthesis of crambin (see structure) with only a single final purification step gave the target protein of exceptional purity in only two days with an overall yield of ≈40 %. Three unprotected peptide segments were linked by native chemical ligation, and the polypeptide chain assumed its 3D structure without intermediate purification steps.

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