Enzyme Microarrays: On-Chip Determination of Inhibition Constants Based on Affinity-Label Detection of Enzymatic Activity

Authors

  • Jörg Eppinger Dr.,

    1. ForschungsDozentur Molekulare Katalyse Lehrstuhl für Anorganische Chemie, Technische Universität München, Lichtenbergstr. 4, 85748, Garching, Germany, Fax: (+49) 89289-13473
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    • These authors contributed equally to this work.

  • Daniel P. Funeriu Dr.,

    1. Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakouji, Amagasaki, Hyogo, 661-0974, Japan, Fax: (+81) 6-6494-7863
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    • These authors contributed equally to this work.

  • Masato Miyake Dr.,

    1. Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakouji, Amagasaki, Hyogo, 661-0974, Japan, Fax: (+81) 6-6494-7863
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  • Lucile Denizot,

    1. Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakouji, Amagasaki, Hyogo, 661-0974, Japan, Fax: (+81) 6-6494-7863
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  • Jun Miyake Dr.

    1. Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakouji, Amagasaki, Hyogo, 661-0974, Japan, Fax: (+81) 6-6494-7863
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  • This work was supported by the Stifterverband für die Deutsche Wissenschaft (Projekt-Nr. 11047: ForschungsDozentur Molekulare Katalyse) and by AIST. We thank Dr. Chikashi Nakamura and Dr. Nicolas Giuseppone for assistance at various stages.

Abstract

original image

High-throughput screening of libraries of potential inhibitors is possible by using an enzyme microarray method that combines low sample consumption with accuracy and speed. Information about enzyme inhibition can be obtained “on-chip” by the addition of a fluorescently tagged affinity label to a microarray of functional enzymes (see picture).

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