Efficient Whole-Cell Biotransformation in a Biphasic Ionic Liquid/Water System


  • We are grateful to Solvent Innovation for providing us with the ionic liquids. This work was supported by the German Federal Ministry of Education and Research (grant no. 0312737E).


original image

Biocompatible ionic liquids act as a substrate reservoir and an in situ extracting agent in biotransformations with whole cells (see scheme). In the reduction of 4-chloroacetophenone to (R)-1-(4-chlorophenyl)ethanol, high product concentration (82 g L−1) and purity (99.6 % ee) were achieved without the addition of cofactors and without damaging the biocatalyst.