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Keywords:

  • analytical methods;
  • enzymes;
  • fluorescence microscopy;
  • kinetics;
  • single-molecule studies
Thumbnail image of graphical abstract

Real-time measurement of the catalysis and substrate kinetics of a single-enzyme hydrolysis reaction is demonstrated with confocal fluorescence microscopy (CFM; see picture, green=CFM beam). A single lipase is shown to have a broad range of conformations; each conformation contributes to the overall enzymatic activity, an observation that is often masked by ensemble measurements.