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Keywords:

  • antibodies;
  • DNA recognition;
  • fluorescent probes;
  • nucleotides;
  • polymerase chain reaction
Thumbnail image of graphical abstract

Antibodies as light switches: The deoxynucleotide analogue–stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene-modified nucleotide. Therefore, DNA generated through PCR with 1 in the substrate pool can be used for hybridization assays in which detection is triggered by the antibody (see scheme).