This work was supported by The Skaggs Institute for Chemical Biology. We thank Dr. Malcolm Wood for technical assistance.
Enzymatic Incorporation of an Antibody-Activated Blue Fluorophore into DNA†
Article first published online: 2 MAR 2005
Copyright © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 44, Issue 14, pages 2144–2148, March 29, 2005
How to Cite
Kaufmann, G. F., Meijler, M. M., Sun, C., Chen, D.-W., Kujawa, D. P., Mee, J. M., Hoffman, T. Z., Wirsching, P., Lerner, R. A. and Janda, K. D. (2005), Enzymatic Incorporation of an Antibody-Activated Blue Fluorophore into DNA. Angew. Chem. Int. Ed., 44: 2144–2148. doi: 10.1002/anie.200461143
- Issue published online: 22 MAR 2005
- Article first published online: 2 MAR 2005
- Manuscript Revised: 22 NOV 2004
- Manuscript Received: 1 JUL 2004
- DNA recognition;
- fluorescent probes;
- polymerase chain reaction
Antibodies as light switches: The deoxynucleotide analogue–stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene-modified nucleotide. Therefore, DNA generated through PCR with 1 in the substrate pool can be used for hybridization assays in which detection is triggered by the antibody (see scheme).