High-Throughput Protein Glycomics: Combined Use of Chemoselective Glycoblotting and MALDI-TOF/TOF Mass Spectrometry

Authors

  • Shin-Ichiro Nishimura Prof.,

    1. Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
    2. Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo 062-8517, Japan
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  • Kenichi Niikura Dr.,

    1. Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
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  • Masaki Kurogochi Dr.,

    1. Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
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  • Takahiko Matsushita,

    1. Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
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  • Masataka Fumoto,

    1. Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo 062-8517, Japan
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  • Hiroshi Hinou Dr.,

    1. Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo 062-8517, Japan
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  • Ryousuke Kamitani,

    1. Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
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  • Hiroaki Nakagawa Dr.,

    1. Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
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  • Kisaburo Deguchi Dr.,

    1. Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
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  • Nobuaki Miura Dr.,

    1. Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
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  • Kenji Monde Dr.,

    1. Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
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  • Hirosato Kondo Dr.

    1. Discovery Research Laboratories, Shionogi & Co. Ltd. Osaka 553-0002, Japan
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  • This work was partly supported by a grant for the National Project on “Functional Glycoconjugate Research Aimed at Developing for New Industry” from the Ministry of Education, Science, and Culture of Japan. Part of this study was presented at the Annual Conference of the Society for Glycobiology, December 3–6, 2003, San Diego, CA (USA).

Abstract

original image

Common oligosaccharides and aldehyde-attached glycoproteins derived from enzymatic modifications can be captured by glycoblotting based on oxylamino-containing polymers. The combination of this glycoblotting and MALDI-TOF/TOF mass spectrometry enabled a high-throughput analytical method to be developed.

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