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Recognizing a Single Base in an Individual DNA Strand: A Step Toward DNA Sequencing in Nanopores

Authors

  • Nurit Ashkenasy Dr.,

    1. Departments of Chemistry and Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-2798
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  • Jorge Sánchez-Quesada Dr.,

    1. Departments of Chemistry and Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-2798
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  • Hagan Bayley Prof. Dr.,

    1. Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK
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  • M. Reza Ghadiri Prof. Dr.

    1. Departments of Chemistry and Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-2798
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  • This work was supported by a grant from the Office of Naval Research (Grant no. MURI-99, N000149910717). We thank W. S. Horne for his generous assistance in molecular modeling studies.

Abstract

original image

The capture of single strands of DNA inside the α-hemolysin (α-HL) transmembrane pore protein upon application of a positive potential leads to single α-HL⋅DNA pseudorotaxane species (see picture). By monitoring the characteristic decreases in the ion conductance of α-HL, a single adenine nucleotide can be identified at a specific location on a strand of DNA.

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