This study was supported by the Fundação para a Ciência e Tecnologia (Lisbon) (FSE-FEDER): project POCTI/QUI/37768/2001 and PhD grant SFRH/BD/1395.
Synthesis of Luciferyl Coenzyme A: A Bioluminescent Substrate for Firefly Luciferase in the Presence of AMP†
Article first published online: 28 APR 2005
Copyright © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 44, Issue 22, pages 3427–3429, May 30, 2005
How to Cite
Fraga, H., Fontes, R. and Esteves da Silva, J. C. G. (2005), Synthesis of Luciferyl Coenzyme A: A Bioluminescent Substrate for Firefly Luciferase in the Presence of AMP. Angew. Chem. Int. Ed., 44: 3427–3429. doi: 10.1002/anie.200462934
- Issue published online: 24 MAY 2005
- Article first published online: 28 APR 2005
- Manuscript Received: 14 DEC 2004
- adenosine monophosphate;
- firefly luciferase;
- total synthesis
Shedding light on luciferase: Luciferyl coenzyme A (1) was synthesized and it was found that luciferase catalyzes light production from luciferyl coenzyme A and AMP. The results of this study open perspectives for new bioanalytical methods and are strong evidence for the evolutionary relationship between firefly luciferase and acyl-CoA synthetases.