Synthesis of Luciferyl Coenzyme A: A Bioluminescent Substrate for Firefly Luciferase in the Presence of AMP

Authors

  • Hugo Fraga Dr.,

    1. LAQUIPAI, Chemistry Department, Faculdade de Ciências da Universidade do Porto, R. Campo Alegre 687, 4169-007 Porto, Portugal, Fax: (+351) 226-082-959
    2. Additional address: Biochemistry Department (U38-FCT), Faculdade de Medicina da Universidade do Porto, 4200-319 Porto, Portugal
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  • Rui Fontes Dr.,

    1. LAQUIPAI, Chemistry Department, Faculdade de Ciências da Universidade do Porto, R. Campo Alegre 687, 4169-007 Porto, Portugal, Fax: (+351) 226-082-959
    2. Additional address: Biochemistry Department (U38-FCT), Faculdade de Medicina da Universidade do Porto, 4200-319 Porto, Portugal
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  • Joaquim C. G. Esteves da Silva Prof. Dr.

    1. LAQUIPAI, Chemistry Department, Faculdade de Ciências da Universidade do Porto, R. Campo Alegre 687, 4169-007 Porto, Portugal, Fax: (+351) 226-082-959
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  • This study was supported by the Fundação para a Ciência e Tecnologia (Lisbon) (FSE-FEDER): project POCTI/QUI/37768/2001 and PhD grant SFRH/BD/1395.

Abstract

original image

Shedding light on luciferase: Luciferyl coenzyme A (1) was synthesized and it was found that luciferase catalyzes light production from luciferyl coenzyme A and AMP. The results of this study open perspectives for new bioanalytical methods and are strong evidence for the evolutionary relationship between firefly luciferase and acyl-CoA synthetases.

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