The Thermal Unfolding of Native Cytochrome c in the Transition from Solution to Gas Phase Probed by Native Electron Capture Dissociation


  • The authors thank the Austrian FWF and BMBWK (grants P15767, T229 to KB) and the NIH (grant GM16609 to FWM) for generous funding, and Huili Zhai, Cheng Lin, Xuemei Han, Robert Konrat, Harry B. Gray, Harold Hwang, and Mi Jin for stimulating discussions.


original image

Unfolding upon dehydration:. What happens to the structure of a native protein that is suddenly exposed to vacuum? Native electron capture dissociation shows that dehydration turns the strongest intramolecular interactions in solution into the weakest in the gas phase.