This work was supported in part by the National Institutes of Health (Grant no.: EB002046) and by the Institute for Collaborative Biotechnologies (Grant no.: DAAD19-03-D-0004) from the U.S. Army Research Office. We gratefully acknowledge Brian Baker and Kevin Cash for providing critical commentary and Professor Pierre Petroff for his support.
Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor†
Article first published online: 26 JUL 2005
Copyright © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 44, Issue 34, pages 5456–5459, August 26, 2005
How to Cite
Xiao, Y., Lubin, A. A., Heeger, A. J. and Plaxco, K. W. (2005), Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor. Angew. Chem. Int. Ed., 44: 5456–5459. doi: 10.1002/anie.200500989
- Issue published online: 22 AUG 2005
- Article first published online: 26 JUL 2005
- Manuscript Received: 17 MAR 2005
- DNA structures;
- electron transfer;
Blue, gold, and DNA: A methylene blue (MB) tagged, thrombin-binding DNA aptamer immobilized on a gold surface undergoes a large conformational change upon target binding (see schematic representation; eT: electron transfer). This folding produces a large, readily measurable change in redox current and allows the electrochemical detection of thrombin in blood serum.