Facile Disulfide Bond Cleavage in Gaseous Peptide and Protein Cations by Ultraviolet Photodissociation at 157 nm


  • This work was supported by the Swedish Research Council and KAW Foundation (to R.Z.) as well as by the CUHK Postgraduate Student Grants for Overseas Academic Activities (grant no. 0680026 to Y.M.E.F.).


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Overall charge unchanged: Disulfide bonds in gaseous polypeptide cations can be cleaved efficiently by ultraviolet photodissociation (UVPD) at 157 nm (see scheme). Unlike electron-capture dissociation, UVPD cleaves S[BOND]S bonds without overall charge reduction in the product ions. This cleavage is suggested to be a result of electronic excitation caused by the absorption of photons of 7.9 eV.