Communication

Facile Disulfide Bond Cleavage in Gaseous Peptide and Protein Cations by Ultraviolet Photodissociation at 157 nm†

  1. Y. M. Eva Fung2,
  2. Frank Kjeldsen Dr.1,
  3. Oleg A. Silivra Dr.1,
  4. T. W. Dominic Chan Prof.2,
  5. Roman A. Zubarev Prof.1

Article first published online: 20 SEP 2005

DOI: 10.1002/anie.200501533

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 44, Issue 39, pages 6399–6403, October 7, 2005

Additional Information

How to Cite

Fung, Y. M. E., Kjeldsen, F., Silivra, O. A., Chan, T. W. D. and Zubarev, R. A. (2005), Facile Disulfide Bond Cleavage in Gaseous Peptide and Protein Cations by Ultraviolet Photodissociation at 157 nm. Angew. Chem. Int. Ed., 44: 6399–6403. doi: 10.1002/anie.200501533

Author Information

  1. 1

    Laboratory for Biological and Medical Mass Spectrometry, BMC, Uppsala University, Box 583, 75123 Uppsala, Sweden, Fax: (+46) 18-471-22-44

  2. 2

    Department of Chemistry, The Chinese University of Hong Kong, H.K.S.A.R., China

  1. †

    This work was supported by the Swedish Research Council and KAW Foundation (to R.Z.) as well as by the CUHK Postgraduate Student Grants for Overseas Academic Activities (grant no. 0680026 to Y.M.E.F.).

Publication History

  1. Issue published online: 30 SEP 2005
  2. Article first published online: 20 SEP 2005
  3. Manuscript Received: 4 MAY 2005

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Keywords:

  • mass spectrometry;
  • photodissociation;
  • proteins;
  • radical chemistry;
  • sequencing
Thumbnail image of graphical abstract

Overall charge unchanged: Disulfide bonds in gaseous polypeptide cations can be cleaved efficiently by ultraviolet photodissociation (UVPD) at 157 nm (see scheme). Unlike electron-capture dissociation, UVPD cleaves S[BOND]S bonds without overall charge reduction in the product ions. This cleavage is suggested to be a result of electronic excitation caused by the absorption of photons of 7.9 eV.

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