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Fluorescence Resonant Energy Transfer Biosensor Based on Upconversion-Luminescent Nanoparticles

Authors

  • Leyu Wang,

    1. Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China
    2. National Center for Nanoscience and Nanotechnology, Beijing, 100084, P.R. China, Fax: (+86) 10-6278-8765
    3. Anhui Key Laboratory of Functional Molecular Solids, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, P.R. China
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  • Ruoxue Yan,

    1. Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China
    2. National Center for Nanoscience and Nanotechnology, Beijing, 100084, P.R. China, Fax: (+86) 10-6278-8765
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  • Ziyang Huo,

    1. Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China
    2. National Center for Nanoscience and Nanotechnology, Beijing, 100084, P.R. China, Fax: (+86) 10-6278-8765
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  • Lun Wang Prof.,

    1. Anhui Key Laboratory of Functional Molecular Solids, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, P.R. China
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  • Jinghui Zeng Dr.,

    1. Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China
    2. National Center for Nanoscience and Nanotechnology, Beijing, 100084, P.R. China, Fax: (+86) 10-6278-8765
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  • Jie Bao,

    1. Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China
    2. National Center for Nanoscience and Nanotechnology, Beijing, 100084, P.R. China, Fax: (+86) 10-6278-8765
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  • Xun Wang Dr.,

    1. Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China
    2. National Center for Nanoscience and Nanotechnology, Beijing, 100084, P.R. China, Fax: (+86) 10-6278-8765
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  • Qing Peng Dr.,

    1. Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China
    2. National Center for Nanoscience and Nanotechnology, Beijing, 100084, P.R. China, Fax: (+86) 10-6278-8765
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  • Yadong Li Prof. Dr.

    1. Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China
    2. National Center for Nanoscience and Nanotechnology, Beijing, 100084, P.R. China, Fax: (+86) 10-6278-8765
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  • This work was supported by the NSFC (90406003, 50372030, 20131030, 20401010), the Specialized Research Fund for the Doctoral Program of Higher Education, the Foundation for the Authors of National Excellent Doctoral Dissertation of P.R. China, and the State Key Project of Fundamental Research for Nanomaterials and Nanostructures (2003CB716901).

Abstract

original image

A pair to fret about: Biotinylated phosphor nanoparticles that emit upconversion luminescence have been taken with biotinylated Au nanoparticles, which act as energy acceptors, and the pair has been applied to the determination of trace amounts of avidin based on fluorescence resonant energy transfer (FRET; see picture).

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