This work was funded by the BBSRC, and the Winston Churchill Foundation provided a studentship to K.T.R.
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Two-Component Graded Deposition of Biomolecules with a Double-Barreled Nanopipette†
Article first published online: 25 OCT 2005
DOI: 10.1002/anie.200502338
Copyright © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Rodolfa, K. T., Bruckbauer, A., Zhou, D., Korchev, Y. E. and Klenerman, D. (2005), Two-Component Graded Deposition of Biomolecules with a Double-Barreled Nanopipette. Angew. Chem. Int. Ed., 44: 6854–6859. doi: 10.1002/anie.200502338
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Publication History
- Issue published online: 25 OCT 2005
- Article first published online: 25 OCT 2005
- Manuscript Received: 5 JUL 2005
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- 3, , Angew. Chem. 2003, 115, 510;Angew. Chem. Int. Ed. 2003, 42, 494.Direct Link:Direct Link:
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- 23The 80- and 160-s dots did not differ significantly in either width or integrated intensity. This indicates that the surface saturation occurs at a deposition time of approximately 80 s (the linear fit mentioned herein was performed with the 160-s data point masked).
- 24Spot sizes have been observed to vary by as much as 50 % between tips. For instance, deposition of IgG at 2.0 V for 10 s yields spot sizes in the range of 500–750 nm depending on the pipette.
- 25Feature sizes reported herein are true spot sizes, deconvoluted with an optical resolution of 350 nm.
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- 27Glass capillaries and pipette pullers for producing tips with up to seven barrels are available from a number of manufacturers: World Precision Instruments Catalog, 2004, 98 and 193.