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Mass Spectrometric Profiling of Intact Biological Tissue by Using Desorption Electrospray Ionization


  • This work was supported by Prosolia Inc. (Indianapolis, IN), the Office of Naval Research, and NIH/NIGMS Grant No. 5R01 GM58008-07. We gratefully acknowledge Pierre Chaurand and Hans-Rudolf Aerni for supplying the mouse pancreas and rat-brain sections and for their technical assistance. In addition, we also thank Anthony Frazier at the Vanderbilt-Ingram Cancer Center, Human Tissue Acquisition and Pathology Shared Resource laboratory for the metastatic human-liver tissue sections.


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Direct chemical profiling of biological tissues, including metastatic human-liver adenocarcinoma, was carried out by using mass spectrometry under ambient conditions. Desorption electrospray ionization revealed enhanced signals from sphingolipids and increased unsaturation levels of the phospholipids in the tumor region of the tissue (see picture).

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