SEARCH

SEARCH BY CITATION

Keywords:

  • enzymes;
  • esterases;
  • lipases;
  • mutagenesis;
  • regiospecificity
Thumbnail image of graphical abstract

A true convert: The carboxylesterase enzyme R.34 (see picture) can be converted into a triacylglycerol lipase without modification of the shape, size, or hydrophobicity of the substrate binding site. The substitution of Asn33 by Asp results in a salt bridge between the Asp33 and Arg49, which causes a distortion of the enzyme structure that makes the catalytic site more accessible to larger substrates but also more labile.