SEARCH

SEARCH BY CITATION

Keywords:

  • enzyme promiscuity;
  • fluorescent probe;
  • medium-chain acyl-CoA dehydrogenase;
  • metabolism;
  • oxidation
Thumbnail image of graphical abstract

Not fussy: A systematic study revealed considerable substrate tolerance of medium-chain acyl-CoA dehydrogenase (MCAD), a member of the β-oxidation pathway for the degradation of fatty acids. Three fluorogenic reporter substrates were developed, which in turn allowed for selective, sensitive, and continuous monitoring of MCAD activity in tissue homogenates.