Fluoromorphic Substrates for Fatty Acid Metabolism: Highly Sensitive Probes for Mammalian Medium-Chain Acyl-CoA Dehydrogenase


  • The authors thank Professor Horst Schulz, Wenfeng Yu, and Lina Nie (City University of New York) for assistance with rat homogenates and supplying the rMCAD enzyme; Professor Jung-Ja Kim (Medical College of Wisconsin) for supplying the pSCAD, pMCAD, and hLCAD enzymes; and Dominic Yee for intellectual contributions. This work was generously supported by The G. Harold & Leila Y. Mathers Charitable Foundation. M.K.F. is the recipient of a NSF Predoctoral Fellowship.


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Not fussy: A systematic study revealed considerable substrate tolerance of medium-chain acyl-CoA dehydrogenase (MCAD), a member of the β-oxidation pathway for the degradation of fatty acids. Three fluorogenic reporter substrates were developed, which in turn allowed for selective, sensitive, and continuous monitoring of MCAD activity in tissue homogenates.