Direct Readout of Protein–Protein Interactions by Mass Spectrometry from Protein–DNA Microarrays


  • The authors thank W. Hecker for help with oligonucleotide synthesis. We are grateful for financial support from the MPG, DFG, BMBF, and VCI.


original image

Catching proteins: Proteins from a protein–DNA microarray can be directly detected by using MALDI mass spectrometry. Capture proteins are attached to silicon chips in a highly selective and reversible way by DNA-directed immobilization (see picture). The analyte proteins bound specifically to their partners on the microarray can be detected from cell lysates.