Direct Readout of Protein–Protein Interactions by Mass Spectrometry from Protein–DNA Microarrays

Authors


  • The authors thank W. Hecker for help with oligonucleotide synthesis. We are grateful for financial support from the MPG, DFG, BMBF, and VCI.

Abstract

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Catching proteins: Proteins from a protein–DNA microarray can be directly detected by using MALDI mass spectrometry. Capture proteins are attached to silicon chips in a highly selective and reversible way by DNA-directed immobilization (see picture). The analyte proteins bound specifically to their partners on the microarray can be detected from cell lysates.

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