Communication

The Transport of Nanosized Gene Carriers Unraveled by Live-Cell Imaging†

  1. Ralf Bausinger1,
  2. Katharina von Gersdorff2,
  3. Kevin Braeckmans Dr.1,3,
  4. Manfred Ogris Dr.2,
  5. Ernst Wagner Prof. Dr.2,
  6. Christoph Bräuchle Prof. Dr.1,
  7. Andreas Zumbusch Priv.-Doz. Dr.1

Article first published online: 7 FEB 2006

DOI: 10.1002/anie.200503021

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 45, Issue 10, pages 1568–1572, February 27, 2006

Additional Information

How to Cite

Bausinger, R., von Gersdorff, K., Braeckmans, K., Ogris, M., Wagner, E., Bräuchle, C. and Zumbusch, A. (2006), The Transport of Nanosized Gene Carriers Unraveled by Live-Cell Imaging. Angew. Chem. Int. Ed., 45: 1568–1572. doi: 10.1002/anie.200503021

Author Information

  1. 1

    Department Chemie und Biochemie and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München, Butenandtstrasse 11, 81377 München, Germany, Fax: (+49) 89-2180-77548

  2. 2

    Department Pharmazie, Ludwig-Maximilians-Universität München

  3. 3

    Current address: General Biochemistry & Physical Pharmacy, Gent University, Belgium

  1. †

    This work was supported by the Deutsche Forschungsgemeinschaft (Grant no. SFB 486). R.B. and K.v.G. contributed equally to this work. K.B. is a Postdoctoral Fellow of the Fund for Scientific Research—Flanders, Belgium.

Publication History

  1. Issue published online: 20 FEB 2006
  2. Article first published online: 7 FEB 2006
  3. Manuscript Received: 24 AUG 2005

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Keywords:

  • DNA;
  • fluorescence microscopy;
  • gene expression;
  • intracellular transport;
  • nanoparticles
Thumbnail image of graphical abstract

Tracking transport: Cationic polyethylenimine nanoparticles serve as nonviral vectors and deliver DNA to the nucleus of cells. Fluorescence live-cell imaging highlights the interactions between these polyplexes and the cytoskeleton during the subsequent stages of transfection (see picture). The analysis of the underlying transport processes gives new impulse to further chemical-synthesis approaches.

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