This work was supported by the Deutsche Forschungsgemeinschaft (Grant no. SFB 486). R.B. and K.v.G. contributed equally to this work. K.B. is a Postdoctoral Fellow of the Fund for Scientific Research—Flanders, Belgium.
The Transport of Nanosized Gene Carriers Unraveled by Live-Cell Imaging†
Article first published online: 7 FEB 2006
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 45, Issue 10, pages 1568–1572, February 27, 2006
How to Cite
Bausinger, R., von Gersdorff, K., Braeckmans, K., Ogris, M., Wagner, E., Bräuchle, C. and Zumbusch, A. (2006), The Transport of Nanosized Gene Carriers Unraveled by Live-Cell Imaging. Angew. Chem. Int. Ed., 45: 1568–1572. doi: 10.1002/anie.200503021
- Issue published online: 20 FEB 2006
- Article first published online: 7 FEB 2006
- Manuscript Received: 24 AUG 2005
- fluorescence microscopy;
- gene expression;
- intracellular transport;
Tracking transport: Cationic polyethylenimine nanoparticles serve as nonviral vectors and deliver DNA to the nucleus of cells. Fluorescence live-cell imaging highlights the interactions between these polyplexes and the cytoskeleton during the subsequent stages of transfection (see picture). The analysis of the underlying transport processes gives new impulse to further chemical-synthesis approaches.