The Transport of Nanosized Gene Carriers Unraveled by Live-Cell Imaging


  • This work was supported by the Deutsche Forschungsgemeinschaft (Grant no. SFB 486). R.B. and K.v.G. contributed equally to this work. K.B. is a Postdoctoral Fellow of the Fund for Scientific Research—Flanders, Belgium.


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Tracking transport: Cationic polyethylenimine nanoparticles serve as nonviral vectors and deliver DNA to the nucleus of cells. Fluorescence live-cell imaging highlights the interactions between these polyplexes and the cytoskeleton during the subsequent stages of transfection (see picture). The analysis of the underlying transport processes gives new impulse to further chemical-synthesis approaches.