L.A.L. acknowledges support from a Sloan Fellowship and a Camille Dreyfus Teacher–Scholar Award.
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Communication
Label-Free Biosensing with Hydrogel Microlenses†
Article first published online: 27 JAN 2006
DOI: 10.1002/anie.200503102
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Kim, J., Singh, N. and Lyon, L. A. (2006), Label-Free Biosensing with Hydrogel Microlenses. Angewandte Chemie International Edition, 45: 1446–1449. doi: 10.1002/anie.200503102
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Publication History
- Issue published online: 13 FEB 2006
- Article first published online: 27 JAN 2006
- Manuscript Received: 31 AUG 2005
Keywords:
- antibodies;
- antigens;
- biosensors;
- gels;
- polymers
Graphical Abstract
Soft lenses: Reversible antibody–antigen cross-links at the surface of a hydrogel microlens allow focal tuning of the microlens in response to soluble antigens (see picture; PBS=phosphate-buffered saline) to form the basis of a label-free biosensor/bioassay technique, whose sensitivity can be tuned by controlling the number of cross-links required to effect a specific swelling response.