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A Fluorescent Indicator To Visualize Activities of the Androgen Receptor Ligands in Single Living Cells

Authors

  • Muhammad Awais Dr.,

    1. Department of Chemistry, School of Science, The University of Tokyo and Japan Science and Technology Agency, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan, Fax: (+81) 3-5841-8349
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  • Moritoshi Sato Dr.,

    1. Department of Chemistry, School of Science, The University of Tokyo and Japan Science and Technology Agency, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan, Fax: (+81) 3-5841-8349
    2. PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, Japan
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  • Xianfen Lee,

    1. Department of Chemistry, School of Science, The University of Tokyo and Japan Science and Technology Agency, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan, Fax: (+81) 3-5841-8349
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  • Yoshio Umezawa Prof. Dr.

    1. Department of Chemistry, School of Science, The University of Tokyo and Japan Science and Technology Agency, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan, Fax: (+81) 3-5841-8349
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  • This work was supported by grants from the Japan Science and Technology Agency (JST) and the Japan Society for the Promotion of Science (JSPS).

Abstract

original image

Seeing the light: An androgen promotes interactions between the androgen-receptor ligand-binding domain (AR LBD) and a coactivator protein. This results in an increase in the fluorescence resonant energy transfer (FRET) from cyan fluorescent protein (CFP) to yellow fluorescent protein (YFP). The indicator distinguishes ligands of different potencies for the AR.

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