Ultrasensitive Detection of Proteins by Amplification of Affinity Aptamers


  • We thank the Natural Sciences and Engineering Research Council of Canada and the Canada Research Chairs Program for financial support of this research.


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Superdetective: The detection of 180 molecules of a protein was achieved by amplification of DNA aptamers that bind to the target protein. The protein–aptamer complex is separated from the unbound aptamer by capillary electrophoresis (CE). The complex fraction is collected and the aptamer amplified by the polymerase chain reaction (PCR). Such amplification dramatically improves the sensitivity of the detection of proteins.