Biosynthesis of the Cyclotide Kalata B1 by Using Protein Splicing

Authors

  • Richard H. Kimura Dr.,

    1. Chemical Biology and Nuclear Sciences Division, Lawrence Livermore National Laboratory, University of California, 7000 East Avenue, Livermore, CA 94550, USA, Fax: (+1) 925-422-3160
    Search for more papers by this author
  • Ahn-Tuyet Tran Dr.,

    1. Chemical Biology and Nuclear Sciences Division, Lawrence Livermore National Laboratory, University of California, 7000 East Avenue, Livermore, CA 94550, USA, Fax: (+1) 925-422-3160
    Search for more papers by this author
  • Julio A. Camarero Dr.

    1. Chemical Biology and Nuclear Sciences Division, Lawrence Livermore National Laboratory, University of California, 7000 East Avenue, Livermore, CA 94550, USA, Fax: (+1) 925-422-3160
    Search for more papers by this author

  • This work was performed under the auspices of the U.S. Department of Energy by the University of California, Lawrence Livermore National Laboratory under Contract W-7405-Eng-48. We thank Dr. David Craik, University of Queensland, Australia, for kindly providing a sample of natural Kalata B1.

Abstract

original image

The tides are turning: Kalata B1 (KB1) is the prototypical member of an exceptionally stable and resistant family of plant-derived miniproteins called cyclotides. The biosynthesis of KB1 and a small library of KB1-based cyclotides (variation occurs in five solvent-exposed loops) in E. coli has been performed by using recombinant-DNA expression techniques and protein splicing.

Ancillary