A General Approach for the Identification of Site-Specific RNA Binders by 19F NMR Spectroscopy: Proof of Concept


  • Financial support from the Austrian Science Fund FWF (P17864 to R.M. and SFB17, project part F1719 to R.K.), the Tiroler Wissenschaftsfonds TWF (GZ: UNI0404/39 to R.M), bm:bwk (GEN-AU project cluster, “Non-coding RNAs”, to R.M.) the Wiener Wissenschafts- und Technologiefonds WWTF (LS162 to R.K.) is gratefully acknowledged.


original image

Bound to succeed: Noncovalent binding of ligands to RNA can be analyzed easily by 19F NMR spectroscopy through site-specific fluorine-labeling of the target RNA (see picture; B=adenine, cytosine, guanine, uracil). The proof of concept is provided by RNA aptamer–ligand interactions. The speed and the robustness of this simple approach present major advantages over methods relying on 1H NMR spectroscopy.