Multiplexed DNA Detection with Biobarcoded Nanoparticle Probes

Authors

  • Savka I. Stoeva,

    1. Department of Chemistry and the International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA, Fax: (+1) 847-467-5123
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    • These authors contributed equally to this work.

  • Jae-Seung Lee,

    1. Department of Chemistry and the International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA, Fax: (+1) 847-467-5123
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    • These authors contributed equally to this work.

  • C. Shad Thaxton,

    1. Department of Chemistry and the International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA, Fax: (+1) 847-467-5123
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  • Chad A. Mirkin Prof.

    1. Department of Chemistry and the International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA, Fax: (+1) 847-467-5123
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  • C.A.M. acknowledges the HSARPA, the DARPA, the AFOSR, the Doris Duke Foundation, and the NIH for support of this research. C.A.M. is also grateful for an NIH Director's Pioneer Award. The authors thank Nanosphere, Inc. for useful discussions and help with microarray construction.

Abstract

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Sorting the mail: A highly selective assay for the detection of multiple DNA targets in solution was developed based on gold nanoparticles with oligonucleotide reporters. Four DNA targets associated with infectious viruses were detected simultaneously without enzymatic amplification at mid-femtomolar concentrations.

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