The authors thank Prof. Ajit Varki for providing plasmids containing the P. damsela α-2,6-sialyltransferase gene in a TOPO vector. This work was supported by Mizutani Foundation for Glycoscience and NIH R01M076360.
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Communication
Highly Efficient Chemoenzymatic Synthesis of Naturally Occurring and Non-Natural α-2,6-Linked Sialosides: A P. damsela α-2,6-Sialyltransferase with Extremely Flexible Donor–Substrate Specificity†
Article first published online: 24 MAY 2006
DOI: 10.1002/anie.200600572
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Yu, H., Huang, S., Chokhawala, H., Sun, M., Zheng, H. and Chen, X. (2006), Highly Efficient Chemoenzymatic Synthesis of Naturally Occurring and Non-Natural α-2,6-Linked Sialosides: A P. damsela α-2,6-Sialyltransferase with Extremely Flexible Donor–Substrate Specificity. Angew. Chem. Int. Ed., 45: 3938–3944. doi: 10.1002/anie.200600572
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Publication History
- Issue published online: 1 JUN 2006
- Article first published online: 24 MAY 2006
- Manuscript Received: 13 FEB 2006
Keywords:
- carbohydrates;
- chemoenzymatic synthesis;
- enzyme catalysis;
- sialic acids;
- transferases
Just relax: A one-pot, three-enzyme chemoenzymatic synthetic approach is highly efficient for obtaining complex sialosides that contain diverse naturally occurring sialic acid modifications. α-2,6-Linked sialosides containing twelve naturally occurring sialic acid forms and seven non-natural sialic acid forms have been obtained.