Highly Efficient Chemoenzymatic Synthesis of Naturally Occurring and Non-Natural α-2,6-Linked Sialosides: A P. damsela α-2,6-Sialyltransferase with Extremely Flexible Donor–Substrate Specificity


  • The authors thank Prof. Ajit Varki for providing plasmids containing the P. damsela α-2,6-sialyltransferase gene in a TOPO vector. This work was supported by Mizutani Foundation for Glycoscience and NIH R01M076360.


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Just relax: A one-pot, three-enzyme chemoenzymatic synthetic approach is highly efficient for obtaining complex sialosides that contain diverse naturally occurring sialic acid modifications. α-2,6-Linked sialosides containing twelve naturally occurring sialic acid forms and seven non-natural sialic acid forms have been obtained.