Constraints on the Structure of (CUG)97 RNA from Magic-Angle-Spinning Solid-State NMR Spectroscopy

Authors

  • Kerstin Riedel,

    1. Research Group Molecular Biophysics/NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstrasse 11, 07745 Jena, Germany, Fax: (+49) 3641-656-225
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  • Christian Herbst,

    1. Research Group Molecular Biophysics/NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstrasse 11, 07745 Jena, Germany, Fax: (+49) 3641-656-225
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  • Sabine Häfner,

    1. Research Group Molecular Biophysics/NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstrasse 11, 07745 Jena, Germany, Fax: (+49) 3641-656-225
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  • Jörg Leppert Dr.,

    1. Research Group Molecular Biophysics/NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstrasse 11, 07745 Jena, Germany, Fax: (+49) 3641-656-225
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  • Oliver Ohlenschläger Dr.,

    1. Research Group Molecular Biophysics/NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstrasse 11, 07745 Jena, Germany, Fax: (+49) 3641-656-225
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  • Maurice S. Swanson Prof. Dr.,

    1. Department of Molecular Genetics and Microbiology, Powell Gene Therapy Center, College of Medicine, University of Florida, 1600 SW Archer Road, Gainesville, FL 32610-0266, USA
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  • Matthias Görlach Dr.,

    1. Research Group Molecular Biophysics/NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstrasse 11, 07745 Jena, Germany, Fax: (+49) 3641-656-225
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  • Ramadurai Ramachandran Dr.

    1. Research Group Molecular Biophysics/NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstrasse 11, 07745 Jena, Germany, Fax: (+49) 3641-656-225
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  • This study has been funded in part by a PhD fellowship to Kerstin Riedel from the Stiftung Stipendien-Fonds des Verbandes der Chemischen Industrie e.V. and a grant from the Deutsche Forschungsgemeinschaft (grant no.: GO474/6-1).

Abstract

original image

Twists in a spin: RNA structural constraints have been obtained directly by exploiting the dependence of inter- and intranucleotide 1H–1H interactions on RNA conformation through the use of 13C MAS solid-state NMR spectroscopy. The data provide evidence that (CUG)97 RNA principally adopts an A-form helix conformation with a C3′-endo sugar pucker and an anti glycosidic torsion angle χ (see model).

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