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Keywords:

  • bioluminescence resonance energy transfer;
  • conjugation;
  • luciferase;
  • protein structures;
  • quantum dots
Thumbnail image of graphical abstract

On the dot: A genetically engineered haloalkane dehalogenase was used to conjugate Renilla luciferase to quantum dots (see picture). The quantum dots can emit light through bioluminescence resonance energy transfer (BRET). This specific conjugation occurs upon simple mixing under mild conditions, and may be applied for specific in vivo labeling of proteins with quantum dots for imaging.