Y. Zhang and M.-K. So equally contributed to this work.
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Communication
HaloTag Protein-Mediated Site-Specific Conjugation of Bioluminescent Proteins to Quantum Dots†
Article first published online: 29 JUN 2006
DOI: 10.1002/anie.200601197
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Zhang, Y., So, M.-k., Loening, A. M., Yao, H., Gambhir, S. S. and Rao, J. (2006), HaloTag Protein-Mediated Site-Specific Conjugation of Bioluminescent Proteins to Quantum Dots. Angew. Chem. Int. Ed., 45: 4936–4940. doi: 10.1002/anie.200601197
- †
This work was supported by the Burroughs Wellcome Fund (to J.R.), a Stanford School of Medicine Dean's Fellowship (to Y.Z.), the Korea Research Foundation Grant M07-2004-000-10234-0 (to M.K.S.), a Stanford Bio-X Graduate Fellowship (to A.M.L.), and the National Cancer Institute Centers of Cancer Nanotechnology Excellence (CCNE) U54.
- ‡
Y. Zhang and M.-K. So equally contributed to this work.
Publication History
- Issue published online: 20 JUL 2006
- Article first published online: 29 JUN 2006
- Manuscript Revised: 19 MAY 2006
- Manuscript Received: 26 MAR 2006
Keywords:
- bioluminescence resonance energy transfer;
- conjugation;
- luciferase;
- protein structures;
- quantum dots
On the dot: A genetically engineered haloalkane dehalogenase was used to conjugate Renilla luciferase to quantum dots (see picture). The quantum dots can emit light through bioluminescence resonance energy transfer (BRET). This specific conjugation occurs upon simple mixing under mild conditions, and may be applied for specific in vivo labeling of proteins with quantum dots for imaging.