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HaloTag Protein-Mediated Site-Specific Conjugation of Bioluminescent Proteins to Quantum Dots

Authors

  • Yan Zhang Dr.,

    1. Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA, Fax: (+1) 650-736-7925
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    • Y. Zhang and M.-K. So equally contributed to this work.

  • Min-kyung So,

    1. Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA, Fax: (+1) 650-736-7925
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    • Y. Zhang and M.-K. So equally contributed to this work.

  • Andreas M. Loening,

    1. Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
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  • Hequan Yao Dr.,

    1. Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA, Fax: (+1) 650-736-7925
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  • Sanjiv S. Gambhir Prof.,

    1. Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA, Fax: (+1) 650-736-7925
    2. Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
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  • Jianghong Rao Prof.

    1. Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA, Fax: (+1) 650-736-7925
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  • This work was supported by the Burroughs Wellcome Fund (to J.R.), a Stanford School of Medicine Dean's Fellowship (to Y.Z.), the Korea Research Foundation Grant M07-2004-000-10234-0 (to M.K.S.), a Stanford Bio-X Graduate Fellowship (to A.M.L.), and the National Cancer Institute Centers of Cancer Nanotechnology Excellence (CCNE) U54.

Abstract

original image

On the dot: A genetically engineered haloalkane dehalogenase was used to conjugate Renilla luciferase to quantum dots (see picture). The quantum dots can emit light through bioluminescence resonance energy transfer (BRET). This specific conjugation occurs upon simple mixing under mild conditions, and may be applied for specific in vivo labeling of proteins with quantum dots for imaging.

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